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Image Search Results
Journal: Molecules
Article Title: Effects of Betaine on LPS-Stimulated Activation of Microglial M1/M2 Phenotypes by Suppressing TLR4/NF-κB Pathways in N9 Cells
doi: 10.3390/molecules24020367
Figure Lengend Snippet: Effects of betaine on LPS-induced TLR4/NF-κΒ signal transduction in N9 microglia cells. Cells were treated with betaine (1 mM) or MIDO (10 μM) for 1 h and then incubated with or without LPS (1 μg/mL) for 24 h. ( A ) Determination of TLR4/Myd88 protein levels by western blot analysis. ( B – D ) Determination of IKK-β, P-IKK-β, IκΒ-α, P-IκΒ-α, NF-κΒ, and p-NF-κΒ protein levels by western blot analysis. MIDO was used as a positive control. Data are presented as the means ± SEM of three independent experiments. The control group included untreated cells. Untreated cells served as a control group. # p < 0.05, compared to the control group; * p < 0.05 and ** p < 0.01, compared to the LPS-treated group.
Article Snippet: Cell extract (50 μg) was applied to a sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, transferred to a polyvinylidene fluoride (PVDF) membrane, and then blocked with PBST containing 5% skimmed milk for 2 h. The membrane was incubated at 4 °C with primary antibodies (TLR4, 1:500; Myd88, 1:1000; iNOS, 1:1000; CD206, 1:2000; Arg-1, 1:1000 [Abcam]; IKK-β, 1:500;
Techniques: Transduction, Incubation, Western Blot, Positive Control